Description: Human VEGFR2 (GenBank Accession No. AF035121), a.a. 805-1356 with N-terminal GST-tag, MW=87 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Synonym(s): KDR, Kinase Insert Domain Receptor, VEGFR2, Vascular Endothelial Growth Factor Receptor 2, CD309, FLK-1, Fetal Liver Kinase 1
Specific Activity: 150 pmol/min/µg
Unit Definition: One unit of KDR activity is defined as the amount of enzyme required to produce 1 pmol of phosphorylated Tyr peptide 1 per min at 37°C.
Assay Conditions: Enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl2, 1 mM EGTA, 200 µM ATP, 0.01% Brij-35, 2 µM substrate (Tyr Peptide 1, Z-lyte kinase assay kit from Invitrogen), and 2 ng enzyme at 37°C for 1 hour.
Formulation: 40 mM Tris pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween, 20% glycerol, 3 mM DTT
Format: aqueous buffer solution
Storage / Stability: >6 months at
Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s): 1. Meyer, M. et al., EMBO J. 18, 363-374 (1999). 2. Rahimi, N. et al., J. Biol Chem 275, 16986- 16992 (2000).
Warning(s): Avoid freeze/thaw cycles
Scientific Category: VEGFR2 (KDR)
Product Type: