Common cleavage tag
The main protelytic cleavage of fusion tags are:
-
Thr (Thrombin)
-
TEV (Tobacco Etch Virus Protease)
-
3C / PreScission (Human Rhinovirus 3C Protease)
-
EKT (Enterokinase)
-
FXa (Factor Xa)
|
Tag |
Sequence and cleavage |
Details |
|
Thr |
LVPR/GS |
Off target cleavage at non-specific sites occurs due to contamination of proteases |
|
TEV |
ENLYFQ/G |
TEV has better specificity compared to EKT, Thrombin or Factor Xa. |
|
3C/PreScission |
LEVLFQ/GP |
PreScission is a highly specific protease. Other name (HRV) 3C or PSP |
|
EKT |
DDDDK/ |
Enterokinase cleaves after the Lysine (K) i |
|
FXa |
IEGR/ |
Other than cleaving after the Arg. FXa’s most common secondary cleavage site is between the Gly and Arg residues in its recognition site |