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Screening and Profiling

West bioscience offers screening and profiling in following drug discovery areas. 

Acetyltransferase Screening and Profiling

GCN5,P300

Bromodomain Screening and Profiling

ATAD2A, BRD2 (BD1 + BD2),BRD4 (BD1 + BD2),CREBBP, ATAD2B,BRD3 (BD1), BRD9 (BD1), SMARCA2, BAZ2B, BRD3 (BD2), BRDT (BD1),TAF1 (BD2), BRD1, BRD3(BD1 + BD2), BRG1, TAF1 (BD1 + BD2), BRD2 (BD1), BRD4 (BD1), BRPF3, TAF1L (BD2), BRD2 (BD2), BRD4 (BD2), CECR2, TAF1L (BD1 + BD2), TRIM24(TIF1)

Cell Surface Receptor Screening

BTLA, CD28, CTLA4, IDO-1, PD-1, PD-L1, PD-L2, PCSK9

DNA Methyltransferase Screening and Profiling

DNMT1, DNMT3A, DNMT3B/DNMT3L

HDAC Screening and Profiling

HDAC 1, HDAC 4, HDAC 7, HDAC 10, HDAC 2, HDAC 5, HDAC 8, HDAC 11, HDAC 3, HDAC 6, HDAC 9

Histone Demethylase Screening and Profiling

FBXL10 (KDM2B), FBXL11 (KDM2A), JARID1A (KDM5A), JARID1B (KDM5B), JARID1C (KDM5C), JHMD1D (KDM7), JMJD1A (KDM3A), JMJD2A (KDM4A), JMJD2B (KDM4B), JMJD2C (KDM4C), JMJD2D (KDM4D), JMJD2E (KDM4DL), JMJD3 (KDM6B) and LSD1 (KDM1A)

Histone Methyltransferase Screening and Profiling

EZH1, EZH2, EZH2 (Y641C), EZH2 (Y641F), EZH2 (Y641H), EZH2 (Y641N), EZH2 (Y641S), G9a, GLP, MLL1 Complex, NSD1, NSD2, PRMT1, PRMT3, PRMT4(CARM1), PRMT5, PRMT6, PRMT8, SETDB1, SET2, SET7/9, SET8, SUV39H1, SMYD2, SUV39H2, and SUV4-20H1

HSP90 Screening

HSP90α (C-terminal), HSP90α (N-terminal), HSP90β (C-terminal), HSP90β (N-terminal)

Immunotherapy Screening

BTLA, CD28, CTLA4, IDO-1, PD-1, PD-L1, PD-L2

Kinase Screening and Profiling

ALK, EGFR (T790M), Her2, BRAF, EGFR (L858R, T790M), Her4, BRAF (V600E), EGFR (murine), Lck, BTK, FAK, Jak2, c-Src, FGFR2,  Jak3, CDK4/CyclinD3, FYN, SYK, EGFR (WT), GSK3a, VEGFR2/KDR, EGFR (L858R), GSK3b

Metabolic Enzymes Screening and Profiling

ACC1, Acetyl Coenzyme A carboxylase 1, IDH1(R132H), Isocitrate Dehydrogenase 1 (R132H), ACC2, Acetyl Coenzyme A carboxylase 2, IDH2 Isocitrate Dehydrogenase 2, ALOX-15, Arachidonate 15-lipoxygenase, IDH2(R140Q), Isocitrate Dehydrogenase 2 (R140Q), ACLY, ATP Citrate Lyase, MTAP, Methylthioadenosine phosphorylase, CA1, Carbonic Anhydrase 1, NAMPT, Nicotinamide phosphoribosyltransferase, CA2, Carbonic Anhydrase 2, NMNAT, Nicotinamide nucleotide adenylyltransferase 1, FUK, Fucokinase, PKL, Pyruvate kinase, Liver, FDH, Formaldehyde dehydrogenase, PKR, Pyruvate kinase, RBC, GK1, Galactokinase, PKM1, Pyruvate kinase M1, IDH1, Isocitrate Dehydrogenase 1, PKM2, Pyruvate kinase M2

Methyl-lysine Reader Screening and Profiling

PARP Screening and Profiling

PARP1 PARP5A (TNKS1), PARP7, PARP11, PARP15, PARP2, PARP5B (TNKS2), PARP8, PARP12, PARP3, PARP6, PARP10, PARP14

PDE Screening and Profiling

PDE1A, PDE4A4B, PDE6C, PDE1B, PDE4B1, PDE7A, PDE1C, PDE4B2, PDE7B, PDE2A1, PDE4C1, PDE8A1, PDE3A, PDE4D2, PDE9A2, PDE3B, PDE4D3, PDE10A1, PDE4A10, PDE4D7, PDE10A2, PDE4A1A, PDE5A, PDE11A

Protease Screening and Profiling

Cathepsin B, Caspase-3, DPP3, A20, HCV1a (D168V), Cathepsin F, Caspase-6, DPP4, Ataxin-3, HCV1b, Cathepsin L, Caspase-7, DPP7, OTUD6Bc, HCV1b (D168V), Cathepsin S, Caspase-8, DPP8, UCHL1, HCV1b (R155K), Cathepsin V, DPP9, UCHL3, HCV1b (R155Q), FAP, USP2, HCV2a, POP, USP5, MME, USP7, MMP1, USP8, MMP2, USP10, MMP9 (Q279R), USP14, Protein C-activated, Renin-activated

PTP Screening and Profiling

CD45/PTPRC,Bas, DEP1/PTPRJ, MEG-1, LAR/PTPRF, MEG-2, PTPIA2//PTPRN, PEST, RPTPβ/PTPRB, PRL-1(2-173), RPTPμ/PTPRM, PRL-2(2-167), RPTPγ/PTPRG, PRL-3(2-173), RPTPσ/PTPRS, PTP-D2/PTPN14, PTP1B(1-321)/PTPN1, PTP1B(1-321)/PTPN1, PTP1B(1-321)/PTPN1, PTP1B (full length), PTPMT1, SHP-1, SHP-2, STYXL1, TC-PTP

Pyruvate Kinase Screening

human and murine PKM2, PKLR Var1 (PKR), PKLR Var2 (PKL), and PKM2 Var2 (PKM1)

Sirtuin Screening and Profiling

Sirtuin 1, Sirtuin 2, Sirtuin 3, Sirtuin 5

 

Stable Cell Lines

Stable Cell Development

   West Bioscience can help you generate stable cell lines that express high level of your target protein at homogeneous levels. 

  The gene of interest will be cloned into your desired mammalian expression vector. The parent cell line (CHO, HEK293 or any your cell lines) will be tested for its sensitivity to antibiotics and optimized for transfection. The stable cell line is selected by appropriate antibiotics. The individual stable clone will be screened by Western Blot for optimal expression 

Applications

  • Stable cell lines for compound screening
  • Reporter Gene assays
  • Cytokine Assays
  • Ion Channel studies

Cell lines in stock

  • AP-1 Reporter – HEK293 Cell Line (JNK signaling pathway)
  • ARE reporter cell line (Nrf2 antioxidant pathway)
  • CFTR – HEK 293 Cell Line
  • CRE/CREB Reporter (Luc) – HEK293 Cell Line (cAMP/PKA Signaling Pathway)
  • GAL4 Reporter (Luc)-HEK293 Cell Line (Glucocorticoid Receptor Pathway)
  • Gli Reporter – NIH3T3 Cell line (Hedgehog Pathway)
  • hERG (Kv11.1) - HEK293 Recombinant Cell line
  • Hippo Pathway TEAD Reporter - MCF7 Cell Line
  • hPDE10A-HEK293 Cell line
  • hPDE1B-HEK293 Cell line
  • hPDE7A-HEK293 Cell Line
  • hPDE7B-HEK293 Cell Line
  • hTMEM16A-HEK293 Cell Line
  • hTRPC3-HEK293 Cell Line
  • IKCA1 (KCNN4) - HEK293 Cell Line
  • ISRE reporter cell line (JAK pathway)
  • Myc Reporter (Luc) – HCT116 Cell Line (Myc Signaling Pathway)
  • NaV1.7–HEK293 Cell Line
  • Nav1.8/β2 - HEK293 Cell Line
  • NF-κB reporter (Luc) - HEK293 Cell line
  • Notch Signaling Pathway Notch1/CSL Reporter - HEK293 Cell Line
  • 100 ml ONE-Step™ Luciferase Assay System
  • PDE7A/CRE Reporter - HEK293 Cell Line
  • RARα Reporter Cell Line
  • RARβ Reporter Cell Line
  • RARγ Reporter Cell Line
  • rPDE7A-HEK293 Cell Line
  • SLC5A5 – HEK293 Recombinant Cell line
  • SRE Reporter - HEK293 Cell Line (ERK Pathway)
  • TCF/LEF Reporter – HEK293 Cell Line (Wnt Signaling Pathway, Lithium-Independent)
  • TCF/LEF reporter-HEK293 cell line (Wnt Signaling Pathway, Lithium-Dependent)
  • TGF/SMAD Signaling Pathway SBE Reporter – HEK293 Cell Line
  • TMEM16B (ANO2) - HEK293 Recombinant Cell line
  • TRPC7-HEK293 Cell Line
  • TRPM2-HEK293 Cell Line
 

Gene Construction

Site-Directed Mutagenesis

   West Bioscience can use either a PCR mutagenesis approach, or an oligonucleotide-directed approach to make site-directed mutagenesis. 

   All mutagenesis clones are fully sequenced. A mini-scale plasmid preparation, and a stab culture of the strain carrying the plasmid, along with a detailed report will be delivered. 

Gene Cloning

   West Bioscience can design and clone your specified gene using PCR amplification from your DNA source (plasmid, or cDNA) or synthetic gene. The gene can be cloned into any plasmid vectors you specified. 

   All final cloned sequences will be fully sequenced to ensure the integrity. A stab culture of the strain carrying the plasmid, along with a detailed report will be delivered.