- Species:Human
- Host Species:E. coli
- MW:71 kDa
|
- Genbank #:NP_660095
- Tag(s):N-terminal SUMO-tag
- a.a:full length
|
| Description: |
Human Praja1 recombinant protein (Genbank Accession No. NP_660095), full length with N-terminal SUMO-tag, expressed in <i>E. coli</i>. |
| Background: |
Praja1 (along with Praja2, 53% identity) is a RING-H2 domain E3 (ubiquitin protein ligase) that is involved in the conjugation of ubiquitin to target substrates along with E1 and E2 enzymes. Substrates for Praja1 include Dlxin-1 (MAGE-D1, human) which regulates Dlx5 dependent transcriptional function (involved in osteoblast differentiation) and ELF and Smad3 involved in the TGF-beta signaling pathway implicated in gastrointestinal cancers. |
| UniProt |
Q8NG27 |
| Synonym(s): |
RNF70, RING finger protein 70 |
| Purity: |
≥85% by SDS-PAGE |
| Biological Activity: |
Typical enzyme concentration of 100 nM - 5 mM is used for in vitro conjugation, depending on conditions. |
| Formulation: |
50 mM Tris Buffer, pH 7.6, 150 mM NaCl, 10% glycerol |
| Format: |
aqueous buffer solution |
| Storage / Stability: |
Stable for ≥1 year at –80°C. |
| Application(s): |
Useful as an E3 ligase in ubiquitin-conjugating reactions and to analyze upregulation/downregulation of E3 enzymes. |
| Reference(s): |
1. Yu, P., et al. PJA1, encoding a RING-H2 finger ubiquitin ligase, is a novel human X chromosome gene abundantly expressed in brain. Genomics, 2002. 79(6): p. 869-74.
2. Mishra, L., et al. The role of PRAJA and ELF in TGF-beta signaling and gastric cancer. Cancer Biol Ther, 2005. 4(7): p. 694-9.
3. Saha, T., et al. RING finger-dependent ubiquitination by PRAJA is dependent on TGF-beta and potentially defines the functional status of the tumor suppressor ELF. Oncogene, 2006. 25(5): p. 693-705.
4. Sasaki, A., et al., A RING finger protein Praja1 regulates Dlx5-dependent transcription through its ubiquitin ligase activity for the Dlx/Msx-interacting MAGE/Necdin family protein, Dlxin-1. J Biol. Chem, 2002. 277(25): p. 22541-6. |
| Warning(s): |
Avoid freeze/thaw cycles. |
| Scientific Category: |
Ubiquitination |
| Product Type: |
Ligases (E3) |
