- Species:Human
- Host Species:Sf9 insect cells
- MW:86 kDa
|
- Genbank #:NM_006154
- Tag(s):N-terminal FLAG-tag
- a.a:150–end
|
Description: |
Human Nedd4 (GenBank Accession No. NM_006154), a.a. 150-end, with N-terminal FLAG-tag, MW= 86 kDa, expressed in a Baculovirus infected Sf9 cell expression system. |
UniProt |
P46934 |
Synonym(s): |
Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, NEDD4 |
Formulation: |
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 80 |
Format: |
aqueous buffer solution |
Storage / Stability: |
>6 months at -80°C. |
Application(s): |
Useful in conjunction with E1 and E2 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
Reference(s): |
1. Kotorashvili A. et al., J. Biol. Chem. 284 (24), 16667-16678 (2009).
2. Plant P.J. et al., Biochem. J. 419 (1), 57-63 (2009). |
Warning(s): |
Avoid freeze/thaw cycles |
Scientific Category: |
Ubiquitination |
Product Type: |
Ligases (E3) |