NEDD4

Description: Human Nedd4 (GenBank Accession No. NM_006154), a.a. 150-end, with N-terminal FLAG-tag, MW= 86 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
UniProt P46934
Synonym(s): Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, NEDD4
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 80
Format: aqueous buffer solution
Storage / Stability: >6 months at -80°C.
Application(s): Useful in conjunction with E1 and E2 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s): 1. Kotorashvili A. et al., J. Biol. Chem. 284 (24), 16667-16678 (2009). 2. Plant P.J. et al., Biochem. J. 419 (1), 57-63 (2009).
Warning(s): Avoid freeze/thaw cycles
Scientific Category: Ubiquitination
Product Type: Ligases (E3)