- Species:Human
- Host Species:Sf9 insect cells
- MW:90.5 kDa
|
- Genbank #:NM_006290
- Tag(s):N-terminal FLAG-tag
- a.a:2–791(end)
|
| Description: |
Human A20, also known as tumor necrosis factor-induced protein 3 (TNFAIP3) or OTU domain-containing protein 7C (OTUD7C), GenBank Accession No. NM_006290, a.a. 2-791(end) with an N-terminal FLAG-tag, MW=90.5 kDa, expressed in Sf9 insect cells via a baculov |
| UniProt |
P21580 |
| Synonym(s): |
A20, tumor necrosis factor-induced protein 3, TNFAIP3, OTU domain-containing protein 7C, OTUD7C |
| Specific Activity: |
0.8 pmol/min/ |
| Assay Conditions: |
Reaction was performed in 50 mM Tris pH 7.4, 1 mM DTT, 0.5 mM EDTA, 500 nM Ub-AMC, and various amount of A20 in a volume of 50 |
| Formulation: |
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 0.05% Tween-20, 20% glycerol, and 80 |
| Format: |
aqueous buffer solution |
| Storage / Stability: |
>6 months at -80°C. |
| Application(s): |
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
| Reference(s): |
1. Shi CS, Kehrl JH. (2010). Autophagy 6(7):986-7.
2. Tavares, R.M., et al. (2010). Immunity 33(2):181-9.
3. Hymowitz, S.G., Wertz, I.E. (2010). Nat. Rev. Cancer 10(5):332-41 |
| Warning(s): |
Avoid freeze/thaw cycles |
| Scientific Category: |
Ubiquitination |
| Product Type: |
Deubiquitylating Enzymes |
