- Species:Human
- Host Species:Sf9 insect cells
- MW:118 kDa
|
- Genbank #:NM_003334
- Tag(s):N-terminal FLAG-tag
- a.a:2–end
|
| Description: |
Human UBE1 (GenBank Accession No. NM_003334), a.a. 2-end, with N-terminal FLAG-tag, MW=118 kDa, expressed in a Baculovirus infected Sf9 cell expression system. |
| UniProt |
P22314 |
| Synonym(s): |
UBE1, UBA1 |
| Assay Conditions: |
UBE1 (100 ng) was added to a reaction mixture containing 4 mM ATP, 2 mM DTT, 5 mM MgCl2, 2.8 |
| Formulation: |
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 80 ng/?l FLAG peptide, 20% glycerol, and 3 mM DTT. |
| Format: |
aqueous buffer solution |
| Storage / Stability: |
>6 months at -80°C. |
| Application(s): |
Useful in conjunction with E2 and E3 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
| Reference(s): |
1. Su Z.L. et al., Leuk. Lymphoma 49 (9), 1821-1822 (2008).
2. Ramser J. et al., Am. J. Hum. Genet. 82 (1), 188-193 (2008). |
| Warning(s): |
Avoid freeze/thaw cycles |
| Scientific Category: |
Ubiquitination |
| Product Type: |
Activating Enzyme (E1) |
