- Species:Human
- Host Species:Sf9 insect cells
- MW:80 kDa
|
- Genbank #:NM_030625
- Tag(s):N-terminal FLAG-tag
- a.a:1418-2136
|
| Description: |
Human Ten-eleven translocation 1 protein (TET1), methylcytosine dioxygenase TET1, Leukemiaassociated protein with a CXXC domain, CXXC-type zinc finger protein 6, GenBank Accession # NM_030625, a.a. 1418-2136(end) with N-terminal FLAG-tag, MW = 80 k |
| UniProt |
Q8NFU7 |
| Synonym(s): |
Ten-eleven translocation 1 protein (TET1), methylcytosine dioxygenase TET1, Leukemiaassociated protein with a CXXC domain, CXXC-type zinc finger protein 6 |
| Assay Conditions: |
TET enzyme was combined with a TET methylatedsubstrate in assay buffer for 90 minutes. A fluorescent-conjugated antibody that recognizes hydroxymethylated product was added and fluorescence was read at excitation: 530 nm and emission 590 nm. |
| Formulation: |
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 80 µg/ml FLAG peptide |
| Format: |
Aqueous buffer solution |
| Storage / Stability: |
>6 months at |
| Application(s): |
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
| Reference(s): |
1. Tahiliani, M., et al., Science. 2009; 324(5929): 930-935.
2. Guo, J.U., et al., Cell. 2011; 145(3): 423- 434. |
| Warning(s): |
Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 |
| Scientific Category: |
TET1, FLAG-tag |
| Product Type: |
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