| Description: | The SBE Reporter kit is designed for monitoring the activity of TGFβ/SMAD signaling pathway in the cultured cells. The kit contains transfection-ready SBE luciferase reporter vector, which is a TGFβ pathway-responsive reporter. This reporter con |
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| Synonym(s): | SBE reporter, TGFβ reporter, TGFbeta reporter, SMAD reporter |
| Supplied As: | Reporter: SBE luciferase reporter vector + constitutively-expressing Renilla luciferase vector Negative Control Reporter: Non-inducible luciferase vector + constitutively-expressing Renilla luciferase vector |
| Concentration: | Both the Reporter and Negative Control Reporter are provided at 60 ng DNA/µl (500 µl each) |
| Format: | assay kit |
| Instructions for use: | See data sheet for detailed transfection and assay protocols. |
| Storage / Stability: | Stable at least 12 months from date of receipt, when stored as directed (-20°C) |
| Application(s): | • Monitor TGF? signaling pathway activity. • Screen for activators or inhibitors of the TGF?/ SMAD signaling pathway. • Study effects of RNAi or gene overexpression on the activity of the TGF? pathway. |
| Reference(s): | Moustakas A et al. (2001) Smad regulation in TGF-beta signal transduction. J.Cell Science. 114(Pt 24): 4359-69. |
| Notes: | The vectors are suitable for transient transfection. They are NOT suitable for transformation and amplification in bacteria. |
| Warning(s): | Avoid freeze/thaw cycles |
| Scientific Category: | SBE Reporter Kit (TGFβ/SMAD signaling pathway) |
| Product Type: | |