- Species:Human
- Host Species:HEK293 Cells, tetracycline-inducible
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- Genbank #:NP_006505.2, NM_004588
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| Description: |
Stable recombinant HEK293 cell line expressing tetracycline-inducible human Nav1.8 (Genbank #NP_006505.2) fused to Green Fluorescent Protein (GFP) [Ex. ~395 nm, 475 nm; em ~510 nm] and the human sodium channel β2 subunit (Genbank #NM_004588; SCN2B). |
| Host Cell line: |
HEK293 Cells |
| Mycoplasma Testing: |
The cell line is confirmed for absence of Mycoplasma species using the PCR-based VenorGeM |
| Format: |
Each vial contains ~1 X 106 cells in 10% DMSO solution |
| Instructions for use: |
See product datasheet for detailed protocol. |
| Storage / Stability: |
Immediately upon receipt, store in liquid nitrogen. |
| Application(s): |
Drug compound screening. Functional assays. Efficient antigen for mouse immunization |
| Reference(s): |
1. Catterall, W.A. et al. Pharmacol. Rev. 57 (4): 397–409 (2005).
2. Muzny, D.M., et al. Nature 440:1194-1198 (2006).
3. Wilson, D.S., et al. Protein Expression and Purification 23 (3): 440–446 (2001). |
| Notes: |
License Disclosure
Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit the use of this cell line in humans or for therapeutic or drug use. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. Publications using this cell line should reference West Bioscience, Inc., San Diego. |
| Amino Acid Sequence: |
A synthetic codon-optimized DNA sequence encoding human Nav1.8 (SCN10A gene) with C-terminal GFP-tag and C-terminal Streptavidin-Binding Peptide (SBP)-tag, and the SCN2B gene encoding human β2 subunit with C-terminal FLAG-tag are stably integrated in tetracycline-inducible HEK293 cells. |
| Scientific Category: |
Recombinant Cell Lines(s) |
| Product Type: |
Ion Channel |
