| Description: | The Gli Reporter |
|---|---|
| Background: | The hedgehog pathway controls stem cell growth in embryonic and adult tissues and promotes tumor growth in a number of human cancers. The mammalian hedgehog proteins, Sonic Hedgehog (Shh), Indian Hedgehog (Ihh), and Desert Hedgehog (Dhh) activate hedgehog signaling by binding to their membrane receptor |
| Synonym(s): | Gli cell line, Hedgehog reporter |
| Supplied As: | Each vial contains ~1.5 X 106 cells in 1 ml of 10% DMSO. |
| Biological Activity: | This cell line is validated for its response to stimulation with murine Sonic Hedgehog and to treatment with inhibitors of the hedgehog signaling pathway. |
| Host Cell line: | NIH3T3 cells (Murine) |
| Mycoplasma Testing: | The cell line has been screened using the PCR-based VenorGeM Mycoplasma Detection kit (Sigma Aldrich) to confirm the absence of Mycoplasma species. |
| Format: | aqueous solution containing DMSO |
| Instructions for use: | See data sheet for detailed culturing and assay protocol. |
| Storage / Stability: | Store in liquid nitrogen immediately upon receipt. |
| Application(s): | • Monitor Hedgehog signaling pathway activity. • Screen for activators or inhibitors of the Hedgehog signaling pathway. |
| Reference(s): | 1. Kinzler KW et al. (1990) Mol Cell Biol. 10(2):634-642. 2. Mullor JL et al. (2002) Trends Cell Biol. 12(12):562-569. 3. Peukert S et al. (2010) ChemMedChem. 5(4):500-512. |
| Notes: | Contact us for pricing. License Disclosure : Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit the use of this cell line in humans or for therapeutic or drug use. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. Publications using this cell line should reference West Bioscience, Inc., San Diego. |
| Warning(s): | Avoid freeze/thaw cycles |
| Scientific Category: | Recombinant Cell Lines(s) |
| Product Type: | Cell Signaling Pathway |