- Species:Human
- Host Species:E. coli
- MW:63.7 kDa
|
- Genbank #:X54131
- Tag(s):N-terminal GST-tag
- a.a:1675–1996
|
| Description: |
Protein tyrosine phosphatase PTPβ (beta), also known as PTPRB (GenBank accession No. X54131), a.a. 1675-1996 (catalytic domain) with N-terminal GST-tag, MW=63.7 kDa, expressed in an <i>E. coli</i> expression system. |
| Background: |
Plays an important role in blood vessel remodeling and angiogenesis. Not necessary for the initial formation of blood vessels, but is essential for their maintenance and remodeling. Can induce dephosphorylation of TEK/TIE2, CDH5/VE-cadherin and KDR/VEGFR-2. Regulates angiopoietin-TIE2 signaling in endothelial cells. Acts as a negative regulator of TIE2, and controls TIE2 driven endothelial cell proliferation, which in turn affects blood vessel remodeling during embryonic development and determines blood vessel size during perinatal growth. Essential for the maintenance of endothelial cell contact integrity and for the adhesive function of VE-cadherin in endothelial cells. |
| Synonym(s): |
PTPB, PTPRB, VEPTP, PTPbeta, RPTbeta, PTPβ |
| Specific Activity: |
57 nmole/min/ |
| Unit Definition: |
One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C. |
| Assay Conditions: |
20 mM HEPES, pH 7.2, 100 mM NaCl, 2 mM EDTA, 1 mM DTT, 1 mM pNPP and PTP?. Assay was done at 30&Deg;C for 5 minutes, and the reaction was monitored at 405 nm continuously. Specific activity was calculated based on ε pNPP= 18,000 M-1cm-1. |
| Formulation: |
25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, 10 mM glutathione |
| Format: |
aqueous buffer solution |
| Storage / Stability: |
>12 months at -80°C. |
| Application(s): |
Useful for the study of enzyme kinetics and regulation, to dephosphorylate target substrates and for screening inhibitors. |
| Reference(s): |
1. I. K. Lund et al., J Biol Chem. 2004, 279: 24226-35.
2. G.H. Peters et al., Biochimie. 2003, 85: 527-34. |
| Warning(s): |
Avoid freeze/thaw cycles |
| Scientific Category: |
Protein Phosphatase |
| Product Type: |
Receptor Tyrosine |
