- Species:Human
- Host Species:FreeStyle 293-F cells
- MW:43 kDa
|
- Genbank #:NM_001908
- Tag(s):C-terminal His-tag
- a.a:CD33 1–16, CathepsinB 18–339
|
| Description: |
Human cathepsin B (GenBank Accession No. NM_001908), CD33 signal peptide (a.a. 1-16) + CathepsinB (a.a. 18-339), with C-terminal His-tag, MW=43 kDa, expressed in FreeStyle 293-F cells. |
| UniProt |
P07858 |
| Synonym(s): |
Amyloid Precursor Protein Secretase, CTSB, APP Secretase |
| Specific Activity: |
≥ 4048 pmol/min/ |
| Assay Conditions: |
25 mM MES, pH 5.0, 10 |
| Formulation: |
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole |
| Format: |
aqueous buffer solution |
| Storage / Stability: |
>6 months at -80°C. |
| Application(s): |
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
| Reference(s): |
1. Tu, C. et al., Cancer Res. 2008 Nov 15;68(22):9147-56.
2. Hwang, JH. et al., Cancer Lett. 2009 Jan 18;273(2):266-72. |
| Warning(s): |
Avoid freeze/thaw cycles |
| Scientific Category: |
Protease(s) |
| Product Type: |
Enzymes |
