- Species:Human
- Host Species:E. coli
- MW:see Description
|
- Genbank #:NM_001229
- Tag(s):C-terminal His-tag
- a.a:1 – 416 (end)
|
| Description: |
Human Caspase 9 (CASP9), GenBank Accession No. NM_001229, a.a. 1 - 416 (end), with C-terminal His-tag, MW=35 &10 kDa, expressed in an <i>E. coli</i> cell expression system. Procaspase-9 is autocleaved between the prodomain and large protease subunit (p35) |
| UniProt |
P55211 |
| Synonym(s): |
Caspase-9, CASP9, Caspase 9, Mch6, ICE-LAP6 |
| Specific Activity: |
≥ 0.11 pmol/min/ |
| Assay Conditions: |
50 mM HEPES, pH 7.2, 50 mM NaCl, 10 mM EDTA, 0.1% Chaps, 5% glycerol, 10 mM DTT, and 10 |
| Formulation: |
50 mM Tris pH 8, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 3 mM DTT, 80 mM imidazole. |
| Format: |
aqueous buffer solution |
| Storage / Stability: |
>6 months at -80°C. |
| Application(s): |
Useful for the study of enzyme kinetics, screening inhibitors,and selectivity profiling. |
| Reference(s): |
1. Guerrero AD., et al., J Clin Cell Immunol.9;3(3) (2012)
2. Luo W., et al., Int J Biol Macromol. S0141-8130(13)00222-5. (2013) |
| Warning(s): |
Avoid freeze/thaw cycles. |
| Scientific Category: |
Protease(s) |
| Product Type: |
Enzymes |
