| Description: | The Fluorogenic DPP3 Assay Kit is designed to measure DPP3 activity using purified DPP3 for screening or profiling applications. The key to the Fluorogenic DPP3 Assay Kit is the specific, fluorogenic substrate. Using this kit, only one simple step on a mi |
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| Background: | Dipeptidyl peptidase-4 (DPP4), also known as adenosine deaminase complexing protein 2, is a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides. DPP4 plays a key role in glucose metabolism, immune regulation, signal transduction and apoptosis. |
| Synonym(s): | DPPIV, CD26, Dipeptidylpeptidase 4, ADCP2 |
| Supplied As: | Supplied in a convenient 96-well format, with purified DPP4 enzyme, DPP substrate, and DPP assay buffer for 100 enzyme reactions. |
| Contraindications: | DMSO >1%, strong acids or bases, ionic detergents, high salt |
| Format: | |
| Instructions for use: | See assay kit data sheet for detailed protocol. |
| Storage / Stability: | At least 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival. |
| Application(s): | Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications. |
| Reference(s): | 1. Deacon CF, Carr RD, and Holst JJ Front Biosci. 2008 Jan 1;13:1780-94. 2. Langley AK, Suffoletta TJ, and Jennings HRPharmacotherapy. 2007 Aug;27(8):1163-80. |
| Notes: | The AMC standard is included so the researcher can quatitatively determine the specific activity of the enzyme using the AMC standard as a measure of how much AMC substrate was cleaved to release free AMC. |
| Warning(s): | Avoid freeze/thaw cycles |
| Scientific Category: | Protease(s) |
| Product Type: | Assay Kits |