| Description: | The PDE10A Assay Kit is designed for identification of PDE10A inhibitors using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by PDE10A to the binding agent. The key to the PDE10A Assay Kit |
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| Synonym(s): | inhibitor screening, assay kit, PDE10A |
| Supplied As: | The PDE10A inhibitor screening assay kit comes in a convenient 96-well format, with purified PDE10A enzyme, fluorescently labeled PDE10A substrate (cAMP), binding agent, and PDE assay buffer for 100 enzyme reactions. |
| Contraindications: | DMSO >1%, strong acids or bases, ionic detergents, high salt |
| Format: | |
| Instructions for use: | See assay kit data sheet for detailed protocol. |
| Storage / Stability: | At least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival. |
| Application(s): | Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications. |
| Reference(s): | 1. Threlfell S, Sammut S, Menniti FS, Schmidt CJ, West AR. J Pharmacol Exp Ther 2009; 328(3):785-95. 2. Menniti FS, Chappie TA, Humphrey JM, Schmidt CJ. Curr Opin Investig Drugs 2007; 8(1):54-9. |
| Notes: | MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED: Fluorescent microplate reader capable to measure fluorescence polarization |
| Warning(s): | Avoid freeze/thaw cycles |
| Scientific Category: | Phosphodiesterase(s) |
| Product Type: | Assay Kits |