- Species:Human
- Host Species:Sf9 insect cells
- MW:103 kDa
|
- Genbank #:NM_015508
- Tag(s):N-terminal GST-tag
- a.a:full length
|
| Description: |
Human PARP7 (GenBank Accession No. NM_015508), full length with N-terminal GST-tag, MW = 103 kDa, expressed in a Baculovirus infected Sf9 cell expression system. |
| UniProt |
Q7Z3E1 Q0PNE2 |
| Synonym(s): |
poly (ADP-ribose) polymerase 7, poly(ADP-ribosyl)transferase 7, PARP7 |
| Unit Definition: |
One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute from NAD into the acid-insoluble form. |
| Assay Conditions: |
Enzyme reaction is conducted using BPSs PARP7 Assay Kit (Catalog #80557) at room temperature for 1 hour. |
| Formulation: |
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 4 mM glutathione, 20% glycerol, and 3 mM DTT. |
| Format: |
aqueous buffer solution |
| Storage / Stability: |
>6 months at -80°C. |
| Application(s): |
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
| Reference(s): |
1. Rouleau M., et al. J Cell Biochem. 2007 Feb 1;100(2):385-401.
2. Augustin A., et al. J Cell Sci. 2003 Apr 15;116(Pt 8):1551-62. |
| Warning(s): |
Avoid freeze/thaw cycles. |
| Scientific Category: |
PARP(s) |
| Product Type: |
Enzymes |
