- Species:Human
- Host Species:E. coli
- MW:52.5kDa
|
- Genbank #:NM_152615, var2
- Tag(s):N-terminal GST-tag
- a.a:221–444(end)
|
| Description: |
Human poly (ADP-ribose) polymerase family, member 15 (PARP15) (GenBank Accession No. NM_152615, var.2), a.a. 221-444(end) with N-terminal GST-tag, MW = 52.5 kDa, expressed in an <i>E. coli</i> expression system. |
| UniProt |
Q460N3 |
| Synonym(s): |
poly (ADP-ribose) polymerase 15, poly(ADP-ribosyl)transferase 15, PARP15 |
| Unit Definition: |
One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute from NAD into the acid-insoluble form. |
| Assay Conditions: |
Enzyme reaction is conducted using BPSs PARP15 Assay Kit (Catalog #80567) at room temperature for 30 minutes. |
| Formulation: |
40 mM Tris pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT and 20% glycerol. |
| Format: |
aqueous buffer solution |
| Storage / Stability: |
>6 months at -80°C. |
| Application(s): |
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
| Reference(s): |
1. Andersson CD., et al. J. Med. Chem. 2012 Sep 13;55(17):7706-18.
2. Ricardo C. T. Aguiar., et al. J. Biochem.2005 Oct 280;(40):33756–65. |
| Warning(s): |
Avoid freeze/thaw cycles. |
| Scientific Category: |
PARP(s) |
| Product Type: |
Enzymes |
