| Description: | The PARP1 Chemiluminescent Activity Assay kit is designed to measure PARP1 activity for screening and profiling applications. The key to the PARP1 Chemiluminescent Activity Assay is the biotinylated substrate. With this kit, only three simple steps are re |
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| Background: | PARP1 is known to catalyze the NAD-dependent addition of poly (ADP-ribose) to histones. |
| Synonym(s): | poly (ADP-ribose) polymerase 1, poly(ADP-ribosyl)transferase, ADPRT, PARP1 |
| Supplied As: | The PARP1 assay kit comes in a convenient 96-well format, with purified PARP1 enzyme, histone mixture, activated DNA, and PARP1 assay buffer for 100 enzyme reactions. |
| Contraindications: | DMSO >1%, strong acids or bases, ionic detergents, high salt |
| Format: | |
| Instructions for use: | See assay kit data sheet for detailed protocol. |
| Storage / Stability: | 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival. |
| Application(s): | Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications. |
| Reference(s): | Brown, J.A., Marala, R.B. J. Pharmacol. Toxicol. Methods 2002 47:137-41. |
| Notes: | This kit is also suitable for use with cell extracts, but nonspecific ribosylation activity may be detected. |
| Warning(s): | Avoid freeze/thaw cycles. |
| Scientific Category: | PARP(s) |
| Product Type: | Assay Kits |