| Description: | The PARP1 Chemiluminescent Activity Assay Kit is designed to measure PARP1 activity for screening and profiling applications. PARP1 is known to catalyze the NADdependent addition of poly (ADP-ribose) to histones. The key to the PARP1 Chemiluminescent A |
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| Synonym(s): | poly (ADP-ribose) polymerase 1, poly(ADP-ribosyl)transferase, ADPRT, PARP1 |
| Supplied As: | The PARP1 assay kit comes in a convenient 384-well format, with purified PARP1 enzyme, histone mixture, activated DNA, and PARP1 assay buffer for 400 enzyme reactions. |
| Contraindications: | DMSO >1%, strong acids or bases, ionic detergents, high salt |
| Format: | |
| Instructions for use: | See assay kit datasheet for detailed protocol. |
| Storage / Stability: | 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival. |
| Application(s): | Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications. |
| Reference(s): | Brown JA, Marala RB. J. Pharmacol. Toxicol. Methods 2002 47:137-41. |
| Notes: | MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED: 1x PBS buffer PBST buffer (1x PBS, containing 0.05% Tween-20) Luminometer or fluorescent microplate reader capable of reading chemiluminescence Adjustable micropipettor and sterile tips Rotating or rocker platform |
| Warning(s): | Avoid freeze/thaw cycles. |
| Scientific Category: | PARP(s) |
| Product Type: | Assay Kits |