PADI2, FLAG-tag

Description: Human PADI2, also known as Peptidyl arginine deiminase, type II, (GenBank Accession No. NM_007365), 1-665(end), with C-terminal FLAG-tag, MW= 77 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
UniProt Q9Y2J8
Synonym(s): PADI-2, Peptidyl arginine deiminase, type II
Specific Activity: ≥19 pmole/min/
Assay Conditions: Enzyme reaction was performed in a buffer (50 mM Tris, pH 7.4, 50 mM NaCl, 10 mM CaCl2, and 2 mM DTT) containing PADI and 10 mM BAEE as a substrate for 30 min at 37°C.
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 80 ng/
Format: Aqueous buffer solution
Storage / Stability: >6 months at –80°C.
Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s): 1. Dong S, et al. (2005.) J. Invest. Dermatol. 124(5):1026-33. 2. Wantanabe Y, et al. (2009). J Hum Genet. 54 (7): 430
Warning(s): Avoid freeze/thaw cycles.
Scientific Category: PADI2, FLAG-tag
Product Type: