- Species:
- Host Species:E. coli
- MW:37.1 kDa
|
- Genbank #:NP_418404
- Tag(s):N-terminal His-FLAG-tags
- a.a:2–321
|
| Description: |
<p>Biotin ligase BirA, GenBank Accession No. NP_418404, a.a. 2 – 321 with N-terminal His-FLAG tag, MW = 37.1 kDa, expressed in an <em>E. coli</em> cell expression system.</p> |
| UniProt |
H0QFJ5 |
| Synonym(s): |
Biotin protein ligase, biotin ligase |
| Assay Conditions: |
BirA (10 |
| Formulation: |
50 mM Tris-HCl, pH 8.0, 50 mM NaCl, 150 mM imidazole, 3 mM DTT, and 5% glycerol. |
| Format: |
aqueous buffer solution |
| Storage / Stability: |
>6 months at |
| Application(s): |
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
| Reference(s): |
1. Li, Y., et al., Protein Expr Purif. 2012 Mar; 82(1): 162-7.
2. Chakravartty, V., et al., J Bacteriol. 2012 Mar; 194(5): 1113-26. |
| Warning(s): |
Avoid freeze/thaw cycles |
| Scientific Category: |
Other Proteins |
| Product Type: |
Other Proteins |
