Description: |
The West Bioscience Site Directed Mutagenesis kit allows for the insertion of multiple mutations into the desired vector in a single reaction. No ligation step is required, saving time and effort. Go from PCR product to transforming cells in under an hour! |
Supplied As: |
Components:
Reagent A
Reagent B
Buffer A
10X BSA (0.1% w/v) |
Efficiency: |
Efficiencies of >90% are typical. |
Format: |
assay kit |
Instructions for use: |
See data sheet for detailed protocol. |
Storage / Stability: |
Kit components may be stored at -20°C or below. Avoid freeze/thaw cycles. Upon first thaw, store all reagents in single use aliquots. Stable for at least 6 months from date of receipt, when stored as directed. |
Application(s): |
The Site-Directed Mutagenesis Kit is designed to insert or "fix" any mutation (point, insert, deletion) into a vector in a quick, single-step reaction. Can be used to add multiple PCR inserts in a single reaction, without time-consuming ligation steps. |
Warning(s): |
Avoid freeze/thaw cycles |
Scientific Category: |
Molecular & Cell Biology |
Product Type: |
Site Directed Mutagenesis |