- Species:Human
- Host Species:Sf9 insect cells
- MW:53.4 kDa
|
- Genbank #:NM_000222
- Tag(s):N-terminal His-tag
- a.a:544–976(end)
|
| Description: |
Human Kit (V654A) (GenBank Accession No. NM_000222), a.a. 544-976(end) with substitution of valine654 to alanine and N-terminal His-tag, MW=53.4 kDa, expressed in an Baculovirus infected Sf9 cell expression system. |
| UniProt |
P10721 |
| Synonym(s): |
KIT, cKIT, c-KIT, PBT, CD117, Piebald Trait, SCFR, Mast/Stem Cell Growth Factor Receptor |
| Specific Activity: |
89 pmol/min/µg |
| Unit Definition: |
One unit is defined as the amount of enzyme that will phosphorylate 1 nmol of tyrosine substrate per minute at pH 7.4 and 30°C. |
| Assay Conditions: |
Enzyme reaction is conducted in a buffer containing 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 |
| Formulation: |
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole |
| Format: |
aqueous buffer solution |
| Storage / Stability: |
>6 months at |
| Application(s): |
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
| Reference(s): |
1. Schumaker, J.A., et al. (2008) J. Clin. Pathol. 61:109-114.
2. Sun, J. et al. (2009) J. Biol. Chem. 284: 11039-11047. |
| Warning(s): |
Avoid freeze/thaw cycles |
| Scientific Category: |
Kinase(s) |
| Product Type: |
Receptor Tyrosine |
