- Species:Mouse
- Host Species:E. coli
- MW:42 kDa
|
- Genbank #:NM_0011949
- Tag(s):N-terminal His-tag
- a.a:full length
|
| Description: |
Mouse ERK2/MAPK2 (K52R) (GenBank Accession No. NM_0011949), full length with mutation on lysine52 to arginine and with an N-terminal His-tag, MW=42 kDa, expressed in <i>E. coli</i> . The K52R mutation prevents ATP binding, making the kinase inactive. |
| Synonym(s): |
ERK2, MAPK1, mitogen-activated protein kinase 1, Extracellular Signal-Regulated Kinase 2, PRKM |
| Specific Activity: |
inactive |
| Formulation: |
45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 125 mM imidazole, 3 mM DTT, 10% glycerol. |
| Format: |
aqueous buffer solution |
| Storage / Stability: |
>6 months at -80°C. |
| Application(s): |
Useful as a substrate for MEK1. |
| Reference(s): |
1. Robinson, M.J., et al. Biochemistry. 1996 May 7;35(18):5641-6.
2. Mitsushima, M. et al. Cell Res. 313 (9), 1830- 1838 (2007).
3. Wille, L. et al. Mol. Immunol. 44 (12), 3085- 3091 (2007). |
| Warning(s): |
Avoid freeze/thaw cycles |
| Scientific Category: |
Kinase(s) |
| Product Type: |
Kinase Substrates |
