| Description: | The BTLA:HVEM[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of BTLA:HVEM signaling. The key to this kit is the high sensitivity of detection of biotin-labeled HVEM by streptavidin-HRP. Only a few simple ste |
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| Background: | The binding of BTLA to the HVEM receptor (herpesvirus entry mediator, also known as TNFRSF14) triggers cell signaling pathways involved in the negative regulation of T cells. BTLA:HVEM interaction plays a key role in the regulation of inflammatory, autoimmune, and antitumor responses, and is an important target for drug discovery. |
| UniProt | Q7Z6A9 |
| Synonym(s): | CD272, CD270, TNFRSF14, ATAR, HVEA, LIGHTR, TR2 |
| Supplied As: | This kit comes in a convenient 96-well format, with biotin-labeled HVEM, purified BTLA, streptavidin-labeled HRP, and assay buffer for 100 binding reactions. |
| Contraindications: | DMSO >1%, strong acids or bases, ionic detergents, high salt. |
| Format: | |
| Instructions for use: | See assay kit data sheet for detailed protocol |
| Storage / Stability: | Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival. |
| Application(s): | This kit is useful for screening inhibitors of HVEM binding to BTLA |
| Reference(s): | 1. Derre L., et al. J. Clin. Invest. 2010, 120(1): 157-167. 2. Steinberg, M.W., et al. J. Exp.Med. 2008, 205(6): 1463-1476. |
| Warning(s): | Avoid freeze/thaw cycles. |
| Scientific Category: | Immunotherapy |
| Product Type: | Assay Kits |