| Description: | Human ERG channel has been stably expressed in HEK293 cell line and its expression was confirmed by Western blotting. The channel activity of hERG was characterized by a fluorescence-based assay using thallium influx as a surrogate indicator of potassiu |
|---|---|
| Supplied As: | Each vial contains ~1.5 X 106 cells in 1 ml of 10% DMSO |
| Biological Activity: | The channel has been validated for activity using a fluorescence-based assay that uses thallium influx as a surrogate indicator of potassium ion channel activity coupled with a thallium-sensitive fluorescent dye. |
| Host Cell line: | HEK293 |
| Mycoplasma Testing: | The cell line has been screened using the PCR-based VenorGeM |
| Format: | aqueous solution containing DMSO |
| Instructions for use: | See data sheet for detailed culturing and assay protocol. |
| Storage / Stability: | Immediately upon receipt, store vials in liquid nitrogen. |
| Reference(s): | 1. Beacham, D.W. et al. (2010). J. Biomol. Screen. 15(4):441-446 2. Murphy, S.M. et al. (2006) J. Pharmacol. Toxicol. Methods. 54(1):42-55 |
| Notes: | CONTACT US FOR A QUOTE License Disclosure Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit the use of this cell line in humans or for therapeutic or drug use. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. Publications using this cell line should reference West Bioscience, Inc., San Diego. |
| Scientific Category: | hERG (Kv11.1) - HEK293 Recombinant Cell line |
| Product Type: | |