| Description: | The Fluorogenic HDAC Class2a Assay Kit is a complete assay system designed to measure histone deacetylase (HDAC) class2a activity for screening and profiling applications. It comes in a convenient 96-well format, with all the reagents necessary for 100 |
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| Background: | HDACs regulate cellular processes by catalyzing the hydrolysis of an acetyl group from acetyllysines in modified proteins. In the HDAC assay, fluorescent-dye molecules are attached to a peptide containing acetyllysine. Attachment to the peptide quenches the fluorescence of the dye. After treatment of the peptide with an HDAC, the reaction is mixed with a development solution that is specific for nonacetylated lysines. If the acetyl group has been removed from the lysine by the HDAC, this solution will release the dye allowing for fluorescence. Fluorescence is therefore directly related to HDAC activity. |
| Synonym(s): | histone deacetylase, HDAC |
| Contraindications: | DMSO >1%, strong acids or bases, ionic detergents, high salt |
| Format: | |
| Instructions for use: | See assay kit data sheet for detailed protocol. |
| Storage / Stability: | 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival. |
| Application(s): | Great for studying enzyme kinetics and screening small molecular inhibitors of HDAC class 2a for drug discovery and HTS applications. This kit is suitable for Class 2a and class 4 HDACs (HDACs 4, 5, 7, 9, 11). |
| Reference(s): | Ontoria, J.M., et al., J. Med. Chem. 2009 Nov 12;52(21):6782-9. 2. |
| Notes: | For class 1 and class 2b HDACs ( HDACs 1, 2, 3, 6, 8, 10), we recommend the individual HDAC assay kits or the Fluorogenic HDAC Assay Kits, Cat. #50033 or #50034. |
| Warning(s): | Avoid freeze/thaw cycles. |
| Scientific Category: | HDAC class 2a Fluorogenic Assay Kit |
| Product Type: | |