| Description: | The JMJD2B Homogeneous Assay Kit is designed to measure activity of the JMJD2B for screening and profiling applications, using AlphaLISA<sup> |
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| Synonym(s): | JHDM3B, KDM4B |
| Supplied As: | The JMJD2B Homogeneous Assay Kit comes in a convenient format, with a 384-well plate, biotinylated histone H3 peptide substrate, primary antibody, demethylase assay buffer, and purified JMJD2B for 384 enzyme reactions. |
| Contraindications: | Avoid green and blue dyes that absorb light in the Alphascreen |
| Format: | |
| Instructions for use: | See assay kit data sheet for detailed protocol. |
| Storage / Stability: | 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival. |
| Application(s): | Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications. |
| Reference(s): | 1. Whetstine JR et al. (2006) Cell 125: 467. 2. Fodor, B.D., Genes & Dev. 2006. 20: 1557-1562. APPLICATION REFERENCE: Nakagawa-Yagi, Y., et al. BMC Complement Altern Med. 12(1):101 (2012). |
| Warning(s): | Avoid freeze/thaw cycles. |
| Scientific Category: | Demethylase(s) |
| Product Type: | Assay Kits |