| Description: | The Fluorogenic HDAC1 Assay Kit is a complete assay system designed to measure histone deacetylase 1 (HDAC1) activity for screening and profiling applications. It comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent |
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| Background: | HDACs regulate cellular processes by catalyzing the hydrolysis of an acetyl group from acetyllysines in modified proteins. In the HDAC assay, fluorescent-dye molecules are attached to a peptide containing acetyllysine. Attachment to the peptide quenches the fluorescence of the dye. After treatment of the peptide with an HDAC, the reaction is mixed with a development solution that is specific for nonacetylated lysines. If the acetyl group has been removed from the lysine by the HDAC, this solution will release the dye allowing for fluorescence. Fluorescence is therefore directly related to HDAC activity. |
| UniProt | Q13547 |
| Synonym(s): | histone deacteylase, HDAC1 |
| Contraindications: | DMSO >1%, strong acids or bases, ionic detergents, high salt |
| Format: | |
| Instructions for use: | See assay kit data sheet for detailed protocol. |
| Storage / Stability: | 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival. |
| Application(s): | Great for studying enzyme kinetics and screening small molecular inhibitors of HDAC1 for drug discovery and HTS applications. |
| Reference(s): | 1. Santo, L., et al., Blood. 2012 Mar 15;119(11):2579-89. 2. Bradner, J.E., et al., Nat Chem Biol. 2010 Mar;6(3): 238-243. |
| Warning(s): | Avoid freeze/thaw cycles. |
| Scientific Category: | Deacetylase(s) |
| Product Type: | Assay Kits |