| Description: | The cAMP/PKA Signaling Pathway CRE/CREB Reporter (Luc) |
|---|---|
| Synonym(s): | CRE cell line, CREB cell line, luciferase reporter |
| Supplied As: | Each vial contains ~1.5 X 106 cells in 1 ml of 10% DMSO. |
| Biological Activity: | This cell line is validated for response to stimulation by Forskolin and to the treatment with an inhibitor of the cAMP/PKA signaling pathway. |
| Host Cell line: | HEK293 cells |
| Mycoplasma Testing: | The cell line has been screened using the PCR-based VenorGeM Mycoplasma Detection kit (Sigma Aldrich) to confirm the absence of Mycoplasma species. |
| Format: | aqueous solution containing DMSO |
| Instructions for use: | See data sheet for detailed culturing and assay protocol. |
| Storage / Stability: | Store in liquid nitrogen immediately upon receipt. |
| Application(s): | • Monitor cAMP/PKA signaling pathway activity. • Screen for activators or inhibitors of the cAMP/PKA signaling pathway. |
| Reference(s): | 1. Montminy, M.R. et al. (1987) Binding of a nuclear protein to the cyclic-AMP response element of the somatostatin gene. Nature 328(6126): 175-178. 2. Fan Chung, K. (2006) Phosphodiesterase inhibitors in airways disease. Eur. J. Pharmacol. 533(1-3): 110-117. 3. Malik, R. et al. (2008) Cloning, stable expression of human phosphodiesterase 7A and development of an assay for screening of PDE7 selective inhibitors. Appl. Microbiol. Biotechnol. 77 (5): 1167-1173. |
| Notes: | Contact us for pricing. License Disclosure : Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit the use of this cell line in humans or for therapeutic or drug use. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. Publications using this cell line should reference West Bioscience, Inc., San Diego. |
| Warning(s): | Avoid freeze/thaw cycles |
| Scientific Category: | CRE/CREB Reporter (Luc) - HEK293 Cell Line (cAMP/PKA Signaling P |
| Product Type: | |