| Description: | The Glucocorticoid Receptor Pathway Reporter Kit is designed for monitoring the activity of the glucocorticoid signaling pathway in cultured cells. The kit contains a transfection-ready expression vector for the glucocorticoid receptor ligand binding doma |
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| Synonym(s): | Glucocorticoid Receptor, GR, Nuclear receptor subfamily 3 group C member 1, NR3C1, GAL4, GAL4/UAS |
| Format: | |
| Instructions for use: | See assay kit datasheet for detailed protocol. |
| Storage / Stability: | >1 year from date of receipt when stored at -20°C |
| Application(s): | |
| Reference(s): | Paguio A, et al. (2010) Improved Dual-Luciferase Reporter Assays for Nuclear Receptors. Curr Chem Genomics. 4: 43-49. |
| Notes: | Materials Required but Not Supplied • Mammalian cell line and appropriate cell culture medium • 96-well tissue culture plate or 96-well tissue culture treated white clear-bottom assay plate (Corning # 3610) • Transfection reagent for mammalian cell line. [We use Lipofectamine™ 2000 (Life Technologies #11668027), however, other transfection reagents should work equally well.] • Opti-MEM I Reduced Serum Medium (Life Technologies #31985-062) • Dual luciferase assay system: Dual-Glo® Luciferase Assay System (Promega #E2920): This system assays cells directly in growth medium. It can be used with any luminometer. Automated injectors are not required. OR Dual-Luciferase® Reporter Assay System (Promega #E1910): This system requires a cell lysis step. It is ideal for luminometers with automated injectors. • Luminometer These vectors are ready for transient transfection. They are NOT SUITABLE for transformation and amplification in bacteria. |
| Warning(s): | Avoid freeze/thaw cycles. |
| Scientific Category: | Cell Based Assay Kits |
| Product Type: | Cell Signaling Pathway |