| Description: | The FOXO Reporter kit is designed to monitor activity of the PI3K/AKT signaling pathway and the transcriptional activity of FOXO proteins in cultured cells. The kit contains the transfection-ready FOXO3 expression vector and the FOXO luciferase reporter v |
|---|---|
| Synonym(s): | FOXO reporter, forkhead reporter, PI3K reporter, AKT reporter, FOXO1 reporter, FOXO3 reporter, FOXO4 reporter |
| Supplied As: | Reporter: FOXO luciferase reporter vector + constitutively-expressing Renilla luciferase vector Negative Control Reporter: Non-inducible luciferase vector + constitutively-expressing Renilla luciferase vector |
| Biological Activity: | This cell line is validated for response to stimulation by Forskolin and to the treatment with an inhibitor of the cAMP/PKA signaling pathway. |
| Concentration: | The Reporter and Negative Control Reporter are provided at 60 ng DNA/µl (500 µl each). The FOXO3 Expression Vector and Negative Control Expression Vector are provided at 100 ng DNA/µl (250 µl each). |
| Format: | assay kit |
| Instructions for use: | See data sheet for detailed transfection and assay protocols. |
| Storage / Stability: | Stable at least 12 months from date of receipt, when stored as directed (-20°C) |
| Application(s): | • Monitor PI3K/AKT signaling pathway activity and FOXO transcriptional activity. |
| Reference(s): | 1. Essaghir, A., et al. (2009). J. Biol. Chem. 284(16):10334-10342 2. Hennessy B.T., et al. (2005). Nat. Rev. Drug Discov. 4(12):988-1004. |
| Notes: | The vectors are suitable for transient transfection. They are NOT suitable for transformation and amplification in bacteria. |
| Warning(s): | Avoid freeze/thaw cycles |
| Scientific Category: | Cell Based Assay Kits |
| Product Type: | Cell Signaling Pathway |