| Description: | The ARE Reporter kit is designed for monitoring the activity of the Nrf2 antioxidant pathway in cultured cells. The kit contains a transfection-ready ARE luciferase reporter vector, which is an Nrf2 pathway-responsive reporter. This reporter contains a fi |
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| UniProt | Q16236 |
| Synonym(s): | ARE reporter, Nrf2 reporter |
| Supplied As: | Reporter: ARE luciferase reporter vector + constitutively-expressing Renilla luciferase vector Negative Control Reporter: Non-inducible luciferase vector + constitutively-expressing Renilla luciferase vector |
| Concentration: | Both the Reporter and Negative Control Reporter are provided at 60 ng DNA/µl (500 µl each) |
| Format: | assay kit |
| Instructions for use: | See data sheet insert for detailed transfection and assay protocols. |
| Storage / Stability: | Stable at least 12 months from date of receipt, when stored as directed (-20°C) |
| Application(s): | • Monitor Nrf2 antioxidant response pathway activity. • Screen for activators or inhibitors of the Nrf2 antioxidant response pathway. • Study effects of RNAi or gene overexpression on the activity of the Nrf2 pathway. |
| Notes: | The vectors are suitable for transient transfection. They are NOT suitable for transformation and amplification in bacteria. |
| Scientific Category: | Cell Based Assay Kits |
| Product Type: | Cell Signaling Pathway |