- Species:Human
- Host Species:E. coli
- MW:71.8 kDa
|
- Genbank #:NM_080685
- Tag(s):N-terminal GST-tag
- a.a:2091–2490
|
| Description: |
Human, recombinant PTP-Bas, catalytic domain (Gene bank accession number NM_080685), a.a. 2091-2490 (catalytic domain), with N-terminal GST tag, MW=71.8 kDa, expressed in an <i>E. coli</i> expression system. |
| Background: |
PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. PTPN13 is a large protein that possesses a PTP domain at C-terminus, and multiple noncatalytic domains, which include a domain with similarity to band 4.1 superfamily of cytoskeletal-associated proteins, a region consisting of five PDZ domains, and a leucine zipper motif. PTPN13 binds to a negative regulatory domain in Fas that inhibits Fas-induced apoptosis. |
| Synonym(s): |
Bas, FAP-1, PTPN13, APO-1/CD95-Associated Phosphatase 1, PTP-BAS, PNP1, PTP1E, PTPL1, PTPLE, PTP-BL, Fas-Associated Phosphatase 1 |
| Specific Activity: |
5 U/ |
| Unit Definition: |
One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C. |
| Assay Conditions: |
The specific activity of Bas was determined using pNPP. Assay conditions: 50 mM HEPES, pH 7.4, 2 mM EDTA, 3 mM DTT, 100 mM NaCl, 20 mM pNPP, and 20 ng/ |
| Formulation: |
25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, 10 mM glutathione. |
| Format: |
aqueous buffer solution |
| Storage / Stability: |
>12 months at -80°C. |
| Application(s): |
Useful for the study of enzyme kinetics and regulation, to dephosphorylate target substrates and for screening inhibitors. |
| Reference(s): |
1. W. Kimber, et al., Biochem.J. 2003, 376: 525-535.
2. N. Kachel, et al. J.Mol. Biol. 2003, 334: 143-155. |
| Warning(s): |
Avoid freeze/thaw cycles |
| Scientific Category: |
Bas (PTPN13) |
| Product Type: |
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