- Species:Human
- Host Species:E. coli
- MW:28.3 kDa
|
- Genbank #:NM_001167
- Tag(s):N-terminal His-tag
- a.a:120–356
|
| Description: |
Human x-linked inhibitor of apoptosis (XIAP), also known as IAP-like protein, API3, X-linked IAP, and BIRC4, a.a. 120 |
| UniProt |
P98170 |
| Synonym(s): |
XIAP, IAP-like protein, API3, X-linked IAP, BIRC4 |
| Assay Conditions: |
50 mM Tris-HCl, pH 7.4, 25 mM NaCl, 0.05% Tween-20, 0.01% BSA, 0.5 nM FAM-SMAC peptide. Incubate 30 minutes at room temperature. Fluorescence polarization is measured at exc 475 nm, em 528 nm. |
| Formulation: |
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM imidazole, and 20% glycerol. |
| Format: |
aqueous buffer solution |
| Storage / Stability: |
>6 months at -80°C. |
| Application(s): |
Useful in binding and inhibition studies of Caspase 9, screening inhibitors, and selectivity profiling. |
| Reference(s): |
1. Nikolovska-Coleska, Z., et al., Anal Biochem. 2008 Mar 1;374(1):87-98.
2. Verhagen, A.M., et al., Cell Death Differ. 2007 Feb;14(2):348-57
3. Fulda, S., Vucic., D., Nat Rev Drug Discov. 2012 Feb 1;11(2):109-24 |
| Warning(s): |
Avoid freeze/thaw cycles |
| Scientific Category: |
Apoptosis |
| Product Type: |
Proteins |
