| Description: | The AP1 Reporter Kit is designed for monitoring the activity of the JNK signaling pathway and the transcriptional activity of AP1 in cultured cells. The kit contains a transfection-ready AP1 luciferase reporter vector. This reporter contains the firefly l |
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| Background: | The stress-activated protein kinase/c-jun N-terminal kinase (SAPK/JNK) family of proteins includes mitogen-activated protein kinases (MAPKs) that are activated by stress, inflammatory cytokines, mitogens, oncogenes, and inducers of cell differentiation and morphogenesis. Upon activation of the SAPK/JNK pathway, MAP Kinase Kinases phosphorylate and activate JNKs. The activated JNKs translocate to the nucleus where they phosphorylate and activate transcription factors such as c-Jun. The activated c-Jun forms homodimers or heterodimers with fos family proteins which bind to the activator protein-1(AP1) response element and induce target gene transcription. |
| Synonym(s): | JNK reporter, SAPK reporter |
| Supplied As: | Reporter: AP1 luciferase reporter vector + constitutively-expressing Renilla luciferase vector Negative Control Reporter: Non-inducible luciferase vector + constitutively-expressing Renilla luciferase vector |
| Concentration: | Both the Reporter and Negative Control Reporter are provided at 60 ng DNA/µl (500 µl each) |
| Format: | assay kit |
| Instructions for use: | See data sheet for detailed transfection and assay protocols. |
| Storage / Stability: | Stable at least 12 months from date of receipt, when stored as directed (-20°C) |
| Application(s): | • Monitor cAMP/PKA signaling pathway activity. • Screen for activators or inhibitors of PKA or cAMP/PKA pathway components. • Study effects of RNAi or gene overexpression on the activity of the cAMP/PKA pathway. |
| Reference(s): | 1. Zhou H. et al. (2005) Frequency and distribution of AP-1 sites in the human genome. DNA Research. 11: 139-150. 2. Gaillard P. et.al. (2005) Design and synthesis of the first generation of novel potent, selective, and in vivo active (benzothiazol-2-yl)acetonitrile inhibitors of the c-Jun N-terminal kinase. J Med Chem. 48(14):4596-4607. |
| Notes: | The vectors are suitable for transient transfection. They are NOT suitable for transformation and amplification in bacteria. |
| Warning(s): | Avoid freeze/thaw cycles |
| Scientific Category: | AP1 Reporter Kit (JNK Pathway) |
| Product Type: | |