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- Clonality:clone FG4R
- Isotype:IgG2b
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| Description: |
Affinity purified Monoclonal Anti-FLAG-Tag, clone FG4R, mouse IgG2b. Recognizes FLAG-tagged (DYKDDDDK) proteins. Applications: Western Blotting (1:1000 - 3000), ELISA, Immunocytochemistry, immunoprecipitation, in situ hybridization (1:500 - 2000). Optimal |
| Synonym(s): |
Antibody, FLAG-tag |
| Formulation: |
1 mg/ml in 10 mM PBS, pH 7.2, with 0.05% NaN3 |
| Cross Reactivity: |
Works in human cells; predicted to work in all proteins containing the FLAG tag, regardless of species. |
| Purification: |
Protein A Affinity Chromatography |
| Sensitivity: |
Recognizes 1-5ng of FLAG tagged proteins in wB with ECL detection. |
| Immunogen: |
Synthetic peptide: DYKDDDDK conjugated to KLH. |
| Format: |
aqueous buffer solution |
| Storage / Stability: |
Store at –20°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| Application(s): |
WB, ICC, IP, ELISA |
| Warning(s): |
Avoid freeze/thaw cycles. |
| Scientific Category: |
Antibodies |
| Product Type: |
Epitope Tag |
