Anti-PD-1 Neutralizing Antibody

Description: Neutralizing recombinant murine/human chimeric antibody recognizing the PD-L1/PD-L2 binding region of human PD-1. This antibody has not been tested for cross-reactivity with other species.
UniProt Q15116
Synonym(s): Anti-PD1
Specific Activity: IC50 = 1.2 nM using the PD-1[Biotinylated]:PD-L1 Inhibitor Screening Assay Kit (BPS Cat. #72005). IC50 = 4.7 nM using the PD-1[Biotinylated]:PD-L2 Inhibitor Screening Assay Kit (BPS Cat. #72006).
Assay Conditions: Antibody was added to PD-1[Biotinylated]:PD-L1 or PD-1[Biotinylated]:PD-L2 binding assay at various concentrations. Assay was performed according to the protocol in BPS Cat. #72005 or #72006, respectively.
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
Purification: Protein A affinity chromatography from HEK293 supernatants.
Immunogen: PD-L1/L2 binding region of human PD-1
Format: Aqueous buffer solution
Storage / Stability: Stable for at least 12 months at -80
Application(s): Useful as a control inhibitor of PD-1 binding using the PD-1:PD-L1[Biotinylated] Inhibitor Screening Assay Kit, West Bioscience, Catalog #72003.
Reference(s): 1. Shinohara, T., et al., Genomics. 1994 Oct;23(3):704-6. 2. James, E.S., et al., Genes Immun. 2005 Aug;6(5):430-7.
Warning(s): Avoid freeze/thaw cycles.
Scientific Category: Anti-PD-1 Neutralizing Antibody
Product Type: