- Species:Human
- Host Species:Sf9 insect cells
- MW:75 kDa
|
- Genbank #:NM_001140
- Tag(s):N-terminal FLAG-tag
- a.a:2– 662(end)
|
| Description: |
ALOX-15, also known as 15LOX-1, omega-6 lipoxygenase, or arachidonate 15-lipoxygenase (GenBank Accession No. NM_001140), a.a. 2- 662(end), with N-terminal FLAG-tag, MW=75 kDa, expressed in Sf9 insect cells via a baculovirus expression system. |
| UniProt |
P16050 |
| Synonym(s): |
15LOX-1, omega-6 lipoxygenase, arachidonate 15-lipoxygenase |
| Assay Conditions: |
ALOX activity was measured using a DPPP (diphenyl-1-Pyrenylphosphine) coupled assay. The enzyme (5.6 ng/ |
| Formulation: |
50 mM Tris-HCl, pH 8.0, 138 mM NaCl, 2.7 mM KCl, and 100 ng/ |
| Format: |
Aqueous buffer solution |
| Storage / Stability: |
>6 months at –80°C |
| Application(s): |
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
| Reference(s): |
1. Jiang, W.G., et al. (2006). Prostaglandins Leukot Essent Fatty Acids. 74 (4): 235-45.
2. Sigal, E., et al. (1988). Biochem. Biophys. Res. Commun. 157 (2): 457-64.
3. Bhattacharya S, et al. (2009). Tumour Biol. 30(4):185-99. |
| Warning(s): |
Avoid freeze/thaw cycles. |
| Scientific Category: |
ALOX15, FLAT-tag |
| Product Type: |
|
