| Description: | The p300 Homogeneous Assay Kit contains an AlphaLISA |
|---|---|
| Background: | The adenovirus E1A-associated cellular p300 transcriptional co-activator protein functions as a histone acetyltransferase that regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. p300 (also known as KAT3B) acetylates all four core histones in nucleosomes, as well as non-histone targets. p300 mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. p300 has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), which plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in this gene are a cause of Rubinstein-Taybi syndrome and its dysfunction is linked to a number of human diseases including Parkinson |
| UniProt | Q09472 |
| Synonym(s): | EP300, KAT3B, RSTS2, E1A binding protein |
| Supplied As: | The p300 Homogeneous Assay Kit comes in a convenient AlphaLISA® format, with biotinylated histone substrate, Acetyl CoA, , primary antibody, assay buffers, and purified p300 for 384 enzyme reactions. |
| Contraindications: | Avoid DMSO concentrations above 0.5%. Avoid green and blue dyes that absorb light in the Alphascreen |
| Format: | |
| Storage / Stability: | Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival. |
| Application(s): | Great for studying enzyme activity and screening small molecular inhibitors for drug discovery and HTS applications. |
| Reference(s): | Trievel, R. C., et al. (2000). Anal. Biochem. 287(2):319-28. |
| Notes: | AlphaScreen® and AlphaLISA® are registered trademarks of PerkinElmer, Inc. |
| Warning(s): | Avoid freeze/thaw cycles |
| Scientific Category: | Acetyltransferase |
| Product Type: | Assay Kit |