| Description: | The Fluorogenic GCN5 Assay Kit is a homogeneous, fluorogenic assay designed to screen for inhibitors of GCN5 (also known as KAT2A). The Fluorogenic GCN5 Assay Kit is based on the transfer of an acetylgroup from acetyl CoA to a peptide substrate. After inc |
|---|---|
| UniProt | Q92830 |
| Synonym(s): | HAT, histone acetyltransferase, GCN5, KAT2A, GCNL2, PCAF-β |
| Supplied As: | The Fluorogenic GCN5 Assay Kit comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent HAT activity measurements. In addition, the kit includes purified GCN5 for use as a positive control. |
| Contraindications: | DTT, β-mercaptoethanol, or other reducing reagents, DMSO >1%, strong acids or bases, ionic detergents, high salt |
| Format: | |
| Instructions for use: | See assay kit data sheet for detailed protocol. |
| Storage / Stability: | 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival. |
| Application(s): | Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications. |
| Reference(s): | Trievel, R. C., et al. (2000). Anal. Biochem. 287(2):319-28. |
| Warning(s): | Avoid freeze/thaw cycles. |
| Scientific Category: | Acetyltransferase |
| Product Type: | Assay Kit |