- Species:Human
- Host Species:Sf9 insect cells
- MW:277 kDa
|
- Genbank #:NM_001093
- Tag(s):N-terminal His-tag
- a.a:full length
|
| Description: |
Human acetyl-Coenzyme A carboxylase alpha transcript variant 2 (ACC2), GenBank Accession No. NM_001093, full length, with N-terminal His-tag, MW=277 kDa, expressed in a Baculovirus infected Sf9 cell expression system. |
| UniProt |
O00763 |
| Synonym(s): |
acetyl-Coenzyme A carboxylase alpha transcript variant 2, ACC-2, ACC 2, ACC -2 |
| Specific Activity: |
≥41 pmoles/min/ |
| Assay Conditions: |
A 25 ?l ACC2 reaction is conducted in a buffer containing 30 mM HEPES (pH 7.4), 2 mM MgCl2, 2 mM potassium citrate, 1 mM DTT, 100 µM ATP, 12 mM NaHCO3 and 20 µM acetyl-CoA at 30 |
| Formulation: |
40 mM Tris pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, and 3 mM DTT. |
| Format: |
Aqueous buffer solution |
| Storage / Stability: |
>6 months at –80°C |
| Application(s): |
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
| Reference(s): |
1. Locke G.A. et al., Arch. Biochem. Biophys. 475 (1), 72-79 (2008).
2. Abu-Elheiga L. et al., Proc. Natl. Acad. Sci. U.S.A. 97 (4), 1444-1449 (2000). |
| Warning(s): |
Avoid freeze/thaw cycles. |
| Scientific Category: |
ACC2, His-tag |
| Product Type: |
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