- Species:Human
- Host Species:Sf9 insect cells
- MW:271 kDa
|
- Genbank #:NM_198834
- Tag(s):C-terminal FLAG-His-tags
- a.a:1–2383(end)
|
| Description: |
Human ACC1 (acetyl-Coenzyme A carboxylase alpha transcript variant 1) (GenBank Accession No. NM_198834), a.a. 1–2383(end) with C-terminal FLAG and His-tags, MW = 270 kDa, expressed in Baculovirus infected Sf9 cell expression system. |
| UniProt |
Q13085 |
| Synonym(s): |
acetyl-Coenzyme A carboxylase alpha transcript variant 1, ACC-1, ACC 1, ACC -1 |
| Specific Activity: |
≥42.5 pmol/min/ |
| Assay Conditions: |
A 25 ?l ACC1 reaction is conducted in a buffer containing 30 mM HEPES (pH 7.4), 2 mM MgCl2, 2 mM potassium citrate, 1 mM DTT, 100 µM ATP, 12 mM NaHCO3 and 20 µM acetyl-CoA at 30 |
| Formulation: |
50 mM Tris-HCl, pH 8.0, 500 mM NaCl, 10% glycerol, 1 mM DTT, 100 |
| Format: |
Aqueous buffer solution |
| Storage / Stability: |
>6 months at –80°C |
| Application(s): |
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
| Reference(s): |
1. Sinilnikova, O.M. et al., Cancer Epidemiol. Biomarkers Prev. 16 (3), 409-415 (2007)
2. Olsen, J.V. et al., Cell 127 (3), 635-648 (2006) |
| Warning(s): |
Avoid freeze/thaw cycles. |
| Scientific Category: |
ACC1, FLAG-His-tags |
| Product Type: |
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