- Species:Human
- Host Species:Sf9 insect cells
- MW:33 kDa
|
- Genbank #:NM_00731
- Tag(s):N-terminal His-tag
- a.a:248–531
|
| Description: |
Human Abelson murine leukemia viral oncogene homolog 1 (Abl-1), GenBank Accession No. NM_005157, a.a. 248-531, with Thr-to-Ile mutation on a.a. 315* and N-terminal His-tag, MW = 33 kDa, expressed in a baculovirus-infected Sf9 cell expression system. *This |
| UniProt |
P00519 |
| Synonym(s): |
Abl-1(T315I), Abl-1(T344I), ABL1, Abelson murine leukemia viral oncogene homolog 1, JTK7 |
| Specific Activity: |
222 pmoles/min/µg. |
| Assay Conditions: |
Activity was determined using a 100% phosphorylated Tyr02 peptide and a Z-lyte kinase assay (Invitrogen). Reaction was incubated at 30°C for 1 hour. |
| Formulation: |
25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol, and 3 mM DTT. |
| Format: |
aqueous buffer solution |
| Storage / Stability: |
>6 months at |
| Application(s): |
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
| Reference(s): |
1. Cao, C., et al. 2003. J. Biol. Chem. 278(15): 12961 |
| Notes: |
This mutation is also cited as T344I in variant B (GenBank Accession No. NM_007313). |
| Warning(s): |
Avoid freeze/thaw cycles |
| Scientific Category: |
Abl Kinase Mutant (T315I) |
| Product Type: |
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