- Species:Human
- Host Species:Sf9 insect cells
- MW:91 kDa
|
- Genbank #:NM_006290
- Tag(s):N-terminal FLAG-tag, C-terminal His-tag
- a.a:2–790(end)
|
| Description: |
Human A20, also known as tumor necrosis factor- induced protein 3 (TNFAIP3) or OTU domain-containing protein 7C (OTUD7C), GenBank Accession No. NM_006290, amino-acids 2-790(end) with an N-terminal FLAG-tag and C-terminal His-tag, MW=91 kDa, express |
| UniProt |
Q9UHC1 |
| Synonym(s): |
tumor necrosis factor-induced protein 3, TNFAIP3, OTU domain-containing protein 7C, OTUD7C, A20 |
| Specific Activity: |
0.766 pmol/min/ |
| Assay Conditions: |
Reaction was performed in 50 mM Tris pH 7.4, 1 mM DTT, 0.5 mM EDTA, 500 nM Ub-AMC, and various amount of A20 in a volume of 50 ?L. Reaction was incubated at 37 |
| Formulation: |
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween- 20, 3 mM DTT, and 20% glycerol |
| Format: |
aqueous buffer solution |
| Storage / Stability: |
>6 months at -80°C. |
| Application(s): |
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
| Reference(s): |
1. Shi CS, Kehrl JH. (2010). Autophagy 6(7):986-7.
2. Tavares, R.M., et al. (2010). Immunity 33(2):181-9.
3. Hymowitz, S.G., Wertz, I.E. (2010). Nat. Rev. Cancer 10(5):332-41 |
| Warning(s): |
Avoid freeze/thaw cycles |
| Scientific Category: |
A20, His-FLAG-tags (Sf9) |
| Product Type: |
|
