- Species:Human
- Host Species:Baculovirus-infected Sf9 insect
- MW:86 kDa
|
- Genbank #:BC_007833
- Tag(s):N-terminal GST-tag
- a.a:Full length
|
| Description: |
Human phosphatidylinositol 4- phosphate 5-kinase type-1 alpha (PIP5K1A) also known as PIP5K1, GenBank Accession No. BC_007833, MW = 86 kDa, full-length expressed in a baculovirus-infected Sf9 insect cell expression system. |
| UniProt |
Q99755 |
| Synonym(s): |
PIP5K1α |
| Specific Activity: |
1310 pmol/min/µg |
| Assay Conditions: |
10 ?l of varying amounts of PIP5K1A was mixed with 5 ?l of 125 µM phosphatidylinositol 3,4-bisphosphate substrate and 5 ?l of kinase dilution buffer. Reaction was initiated by adding 5 ?l of 250 µM ATP assay solution, sonicating for 10 sec. and incubating for 15 min at 30 |
| Formulation: |
50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol. |
| Format: |
Aqueous buffer solution |
| Storage / Stability: |
>6 months at -80 |
| Application(s): |
Useful for studying enzyme kinetics and screening small molecule inhibitors. |
| Reference(s): |
1. Pan, W., et al., Science. 2008; 321: 1350-1353. 2. Barrero-Villar, M., et al., J. Immunol. 2008; 15;181(10): 6882-6888. |
| Warning(s): |
Avoid freeze/thaw cycles. Storing diluted protein is not recommended, if necessary, use carrier protein (BSA 0.1 |
| Scientific Category: |
PIP5KA, GST-tag |
| Product Type: |
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