Description: Human phosphatidylinositol 4- phosphate 5-kinase type-1 alpha (PIP5K1A) also known as PIP5K1, GenBank Accession No. BC_007833, MW = 86 kDa, full-length expressed in a baculovirus-infected Sf9 insect cell expression system.
UniProt Q99755
Synonym(s): PIP5K1α
Specific Activity: 1310 pmol/min/µg
Assay Conditions: 10 ?l of varying amounts of PIP5K1A was mixed with 5 ?l of 125 µM phosphatidylinositol 3,4-bisphosphate substrate and 5 ?l of kinase dilution buffer. Reaction was initiated by adding 5 ?l of 250 µM ATP assay solution, sonicating for 10 sec. and incubating for 15 min at 30
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.
Format: Aqueous buffer solution
Storage / Stability: >6 months at -80
Application(s): Useful for studying enzyme kinetics and screening small molecule inhibitors.
Reference(s): 1. Pan, W., et al., Science. 2008; 321: 1350-1353. 2. Barrero-Villar, M., et al., J. Immunol. 2008; 15;181(10): 6882-6888.
Warning(s): Avoid freeze/thaw cycles. Storing diluted protein is not recommended, if necessary, use carrier protein (BSA 0.1
Scientific Category: PIP5KA, GST-tag
Product Type: