| Description: | The PD-1:PD-L2 TR-FRET Assay Kit is designed to measure the inhibition of PD-1 binding to PD-L2 in a homogeneous 384 reaction format. Cell signaling through the PD-1 receptor upon binding the PD-L2 ligand attenuates immune responses and is exploited by |
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| Supplied As: | This kit comes in a convenient 384-well format, with Europium-labeled PD-1 and biotin-labeled PD-L2. |
| Contraindications: | DMSO >1%, strong acids or bases, ionic detergents, high salt. |
| Format: | |
| Instructions for use: | See product datasheet for detailed protocol. |
| Storage / Stability: | Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival. |
| Application(s): | Great for screening small molecular inhibitors for drug discovery and HTS applications. |
| Reference(s): | 1. Molnar, E., et al. Proc Natl Acad Sci USA. 2008; 105: 10483-10488. 2. Keir, M.E., et al. Annu. Rev. Immunol. 2008; 26: 677-704. |
| Notes: | MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED: Fluorescent microplate reader capable of measuring Time Resolved Fluorescence Resonance Energy Transfer (TR-FRET) Adjustable micropipettor and sterile tips |
| Warning(s): | Avoid freeze/thaw cycles |
| Scientific Category: | PD-1:PD-L2 TR-FRET Assay Kit |
| Product Type: | |