| Description: | The PD-1:PD-L2 Homogeneous Assay Kit is designed to measure the inhibition of PD-1 binding to PD-L2. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing PD-1 and an inhibitor of choice is incubated with |
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| Synonym(s): | Programmed cell death 1, PDCD1, PD-1, PD1, SLEB2, CD279, HPD-L and Programmed Cell Death 2 Ligand 2, PDL2, PD-L2, CD273, PDCDL1G2, B7-DC |
| Supplied As: | The PD-1:PD-L2 Homogeneous Assay Kit comes in a convenient AlphaLISA |
| Contraindications: | Only limited amounts of DMSO can be included, as it has been shown to disrupt PD-1:PD-L2 interaction. Avoid green and blue dyes that absorb light in the AlphaScreen signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+, Zn2+ and Ni2+). The presence of >1% RPMI 1640 culture medium leads to a signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect AlphaScreen assays. |
| Format: | |
| Instructions for use: | See assay kit data sheet for detailed protocol |
| Storage / Stability: | Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival. |
| Application(s): | Useful for screening for inhibitors of PD-1 binding to PD-L2 |
| Reference(s): | 1. Molnar, E. et al. Proc. Natl. Acad. Sci. U.S.A. 2008; 105: 10483-10488 2. Keir, M.E. et al. Annu. Rev. Immunol. 2008; 26: 677-704. |
| Warning(s): | Avoid freeze/thaw cycles |
| Scientific Category: | PD-1:PD-L2 Homogeneous Assay Kit |
| Product Type: | |