||The PD-1:PD-L1 Homogeneous Assay Kit is designed to measure the inhibition of PD-1 binding to PD-L1. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing PD-1 and an inhibitor of choice is incubated with
||Programmed cell death 1, PDCD1, PD-1, PD1, SLEB2, CD279, HPD-L, Programmed Cell Death 1 Ligand 1,CD274 and B7 homolog 1 (B7-H1), PDCD1 ligand 1, programmed death ligand 1, PDCD1L1
||The PD-1:PD-L1 Homogeneous Assay Kit comes in a convenient AlphaLISA
||Only limited amounts of DMSO can be included, as it has been shown to disrupt PD-1-PD-L1 interaction. Avoid green and blue dyes that absorb light in the AlphaScreen signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+, Zn2+ and Ni2+). The presence of >1% RPMI 1640 culture medium leads to a signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect AlphaScreen assays.
|Instructions for use:
||See product datasheet for detailed assay protocol.
|Storage / Stability:
||At least one year from date of receipt when stored as directed.
||Useful for screening for inhibitors of PD-1 binding to PD-L1
||1. Lin, D., et al. Proc Natl Acad Sci U.S.A. 2008, 105: 3011-3016.
2. Keir, M.E. et al. Annu. Rev. Immunol. 2008, 26: 677-704.
||MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED:
AlphaLISA FLAG acceptor beads, 5 mg/ml (PerkinElmer #AL112C)
AlphaScreen Streptavidin-conjugated donor beads, 5 mg/ml (PerkinElmer #6760002S)
Optiplate -384 (PerkinElmer #6007290)
AlphaScreen microplate reader
Adjustable micropipettor and sterile tips
||Avoid freeze/thaw cycles.
||PD-1:PD-L1 Homogeneous Assay Kit